Coding

Part:BBa_K4619005

Designed by: KaMing Li   Group: iGEM23_UCAS-China   (2023-10-09)


1amb-IMME3

This part codes for the engineered antidote ImmE3 for colicin E3(colE3, BBa_1631003). ImmE3 could neutralize the toxicity by forming a ColE3-ImmE3 complex and plays a critical role in plasmid maintenance.

- An amber stop codon is inserted next to the AUG start codon. Only when applying the engineered aminoacyl-tRNA synthase and unnatural amino acid will the antidote be expressed (though there could still be leakage, it could be manipulated by controlling the concentration). Otherwise, the translational could be directly terminated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None